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KMID : 1151820120060060507
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Journal of the Korean Society of Radiology
2012 Volume.6 No. 6 p.507 ~ p.513
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A Research on Superparamagnetic Iron Oxide Nanoparticles' Toxicity to U373MG Cell and its Effect on the Radiation Survival Curve
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Kang Seong-Hee
Kim Jeong-Hwan
Kim Do-Kyung
Kang Bo-Sun
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Abstract
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This research was performed to evaluate the superparamagnetic iron oxide nanoparticles’(SPIONs) cell toxicity and to measure the radiation cell survival curve changes of SPIONs-uptake glioblastoma multiforme cells. The results could be practically used as the fundamental data to ameliorate proton beam cancer therapy, for example, providing necessary GBM treatment dose in the proton beam therapy when the therapy takes advantage of SPIONs. The assessment of the toxicological evaluation of synthesized SPIONs was accomplished by MTT assay as an in vitro experiment. The results showed no meaningful differences in the cell survival rate at the 1-100㎍/ml SPIONs concentrations, but the cell toxicity was shown as the cell survival rate decreased up to 74.2% at the 200㎍/ml SPIONs concentration. Then, we measured each radiation cell survival curve for U373MG cells and SPIONs-uptake U373MG cells with 0~5 Gy of proton beam irradiations. It is learned from the analysis of the experimental results that the SPION-uptake cells’ radiation survival rate was more rapidly decreased as the irradiation dose increased. In conclusion we confirmed that SPIONs-uptake in U373MG cells induces cell death at the much less dose than the lethal dose of SPION-non-uptake cell. This research shows that the therapeutic efficacy of glioblastoma multiforme treatment in proton beam therapy can be improved by SPIONs targeting to the GBM cells.
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KEYWORD
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Proton beam, GBM, Cell toxicological evaluation, MTT assay, Cell survival curve, U373MG cell, Superparmagnetic Iron Oxide Nanoparticles, SPIONs
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